ISF4-X (Climo Shaker) 

Product

ISF4-X large

Product

Large incubator shaker with a minimal footprint and up to five independent shaker units

Description

The Kuhner ISF4-X incubator shaker offers large shaking capacity with temperature, humidity and CO2 control all available. The shaker cabinet can incorporate four or even five independent shaking units which are height adjustable. The shaking diameter and speed of each unit can be individually selected. Standard diameters are 12.5, 25 and 50 mm. Other shaking diameters such as 3mm, 6mm, 19mm and 70mm are possible.

Capacity

The ISF4-X handles more cultures per footprint than any shaker combination in the market. 60L of culture is possible when configured three shaking shelves. Add a fourth shelf to achieve 160 deep well plates or 320 low well plates all using System Duetz.

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Performance Characteristics

  • All Stainlees-steel interior
  • Large Shaking Capacity
  • Each shaking unit has own drive and is adjustable diameter
  • Heating and cooling control
  • CO2 and dual-sided humidity control options available
  • Every machine rigorously calibrated prior to shipment

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Guaranteed Quality

When designing the ISF4-X special emphasis was placed on reliability and durability. The high quality of Kuhner shaking machines is backed with a five year warranty.

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Safety and Convenience

The large door opening of the ISF4-X allows easy access to the shake flasks inside. The doors are fitted with a safety switch to ensure shaking stops when the doors are opened. 

The large windows allow a clear view of incubator’s contents. The entire ISF4-X chamber is clad in stainless steel making sterilization with chemicals possible.

 

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Energy saving operation

The highly efficient direct drive system means economic low power consumption and the cooling system also operates with energy efficiency.

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Electrical protection

Automatic supervision of the mains voltage protects the shaker and in the even of a voltage spike the unit disconnects from the mains.  The machine resumes operation at the set values once power is restored.

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Technical Data

Technical data

Stackable incubator shaker for microbial, mammalian and plant cell applications.

Technical data

 

SMX1600

SMX1601

SMX1603 

Refrigerator   no yes  yes
Humidity control   no no yes 
Temperature minimum   ambient +10oC  ambient - 15oC ambient -15o
Temperature maximum 80oC 80oC 80oC
Humidity maximum - - 85% r.h.
Power consumption < 1700W < 2000W < 2600W

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Machine

Volume (empty)  800 l
Weight (with cooling)  520 kg
Illumination  2 fl lamps
Operation menu in German, French, English, Italian, Spanish
Interface, standard CAN-Bus
Interface, optional USB, Ethernet, digital, analogue
Ambient temperature

10oC up to 35oC

Dimensions (in mm)

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Temperature

Setting, digital  0.1oC
Accuracy, absolute  +/- 0.30oC (37oC)
Principle of sensor  Pt-100
Power of heating 1000W
Power of cooling 250...420W
Air circulation

700m3/h

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Humidity

 

SMX1603

Max at 25...55°C 85% r.h.
Setting, digital 1% r.h.
Accuracy, absolute +/- 2% r.h.
Principle of sensor capacitive
Water refill

automatic

Water heater

300W

Door heater 220W

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CO2

 

SM1034

Principle of sensor Infrared, NDIR
Measuring range 0...20% CO2
Setting, digital 0.1% 
Accuracy, absolute +/- 0.42% at 20% CO2
Non-linearity <0.1%
Temperature range 5...60oC
CO2 supply max. 1 bar overpressure

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Mains Connection

SMX1021 220-240V / 50-60 Hz
SMX1022 190-210V / 50-60 Hz

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Shaking Unit SF-X

 

SMX1610

Tray, size F (800x420 mm)
Loading, maximum 25 kg
Setting, digital 1 rpm
Accuracy, absolute +/- 0.1 rpm
Timer 1 s - 999 h
Acceleration controlled
Active brake adjustable
Stop on position adjustable

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Shaking Motion

Standard machines have adjustable orbit diameters and 12.5mm, 25mm and 50mm are included with every instrument. Optional 19mm (3/4") and 70mm orbit diameters may be added at any time, to any machine.

  Speed
* orbital, Ø 12.5mm 20...500rpm
* orbital,Ø 19mm 20...450rpm
* orbital, Ø 25mm 20...400rpm
* orbital, Ø 50mm 20...300rpm
* orbital,Ø 70mm 20...200rpm
* linear, Ø 12.5mm 20...400rpm
* linear, Ø 25mm 20...300rpm
* linear, Ø 50mm 20...200rpm
* can be changed  

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Options

Options

Large incubator shaker with a minimal footprint and up to five independent shaker units.

Cooling

All Kuhner incubator shakers can be fitted with cooling. The refrigeration system is regulated by a fully digital PID control ensuring minimal energy consumption. The cooling is switched on only when required as determined by the chamber temperature and set point.

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Humidity control

Humidity control is an important factor in successful fermentation. Evaporation from microtiter plates, or when cultivating in flasks for long periods (e.g. cell cultures), can be significantly reduced with humidification.

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CO2 control

CO2 control is essential when working with mammalian cell cultures. Kuhner was the first company to manufacture and supply incubator shakers with CO2 control more than ten years ago and continues to offer this option.

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Pull-out table

The pull-out table is designed for the ISF1-X and ISF4-X incubator shakers as well as the Rack System. This remarkable device makes loading and unloading trays much easier.

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Dual table

The dual table is an easy and economical way of doubling the shaking capacity. The dual shaking table consists of two levels. Each level will accept an E, EX or F size tray. However, with the dual table the shaking speed is limited to a maximum of 200 rpm.

IMG 1138

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Illumination unit for photosynthesis (LED)

The ceiling of any Kühner incubator shaker can be fitted with LED modules for the cultivation of phototrophic organisms. The control module allows full programming of night/day cycles and variable light intensity.

DSC00105

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UV lamp

As in a biological safety cabinet, the chamber of a Kuhner incubator shaker can also be sterilized with an integrated UV lamp. The lamp is situated to maximize irradiation of the chamber and shaking table. The UV lamp has a clearly marked external switch and when the door is opened, the UV-lighting is automatically switched off.

DSC00109

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Additional integrated UV lamp

In the space between the incubator chamber and the rear outer wall of the ISF4-X, a UV lamp can be mounted.  This can be used while shaking to decrease the number of germs in the circulated air.

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Black window

Available for light sensitive medium or organisms. Any Kuhner incubator shaker can be delivered with blackened windows to prevent unwanted daylight or UV radiation inside the incubator.

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Perspex cover

To prevent accidental operation of the controllers, the panel can be protected by a perspex cover.

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BPM-60

BPM-60 (Bioprocess Monitoring) is a non-invasive, online measurement of dissolved oxygen and pH in an Erlenmeyer flask.

Continuous, recording of dissolved oxygen and pH

Dissolved oxygen and pH can be easily measured online using the BPM-60. The system allows for one or both parameters to be recorded in the same flask. The values of dissolved oxygen and/or pH can be monitored simultaneously in eight different flasks.

The continuous data logging, compared to conventional sample taking, is automatic and consistent. The recorded data can be analyzed to identify oxygen limitation or inhibition and therefore optimize yields in shaken flasks.

Furthermore, the BPM-60 assists in the production of reproducible starter cultures for use in fermenters (see PAT-initiative of the FDA).

Not on battery mode

An integrated socket at the front of the shaking table makes simple data communication and power supply possible without the risk of wiring breaking. With this technology no battery is required.

ISIS software for data logging

Kuhner ISIS Software is capable of data recording. In addition to logging dissolved oxygen and pH it is also possible to record all other shaker parameters such as shaking speed and CO2 concentration. A full audit trail is also stored for each shaker connected.

bpm collage

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IQ-OQ documentation

IQ-OQ (Installation Qualification and Operation Qualification) is an equipment qualification according to GMP procedure. Kuhner offers this service which can also be carried out at the customer’s premises.

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Accessories

Accessories

Stackable incubator shaker for microbial, mammalian and plant cell applications.

 

 

Universal tray FU

Order number: SM3003

The universal tray can be fitted with clamps for Erlenmeyer flasks, test tube
holders, microtiter plates, or custom made holders to suit your requirements.
Rearranging these clamps and holders is quick and simple.

  • Clamps for Erlenmeyer flasks
  • Test tube holders
  • High capacity tube holders
  • Holders for deep well microtiter plates (Duetz System)
  • Sticky mats

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F-tray (800 x 420mm) with fixed clamps

The clamps are riveted onto this tray and cannot be removed. It offers maximum stability and will often hold a larger number of flasks than an universal tray.

Order Number Description Number of Clamps
SM330025 F-25 ml 153
SM330050 F-50 ml 100
SM330100 F-100 ml 74
SM330125 F-125 ml 60
SM330150 F-150 ml 60
SM330200 F-200 ml 40
SM330250 F-50 ml 40
SM330300 F-300 ml 30
SM330500 F-500 ml 26
SM331000 F-1000 ml 16
SM331500 F-1500 ml 12
SM332000 F-2000 ml 9
SM333000 F-3000 ml 8
SM334000 F-4000 ml 5
SM335000 F-5000 ml 4

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F-tray (800 x 420mm) for microtiter plates

Kuhner has designed trays specifically for microtiter plates. Handles at the front of the tray open and close the special clamping mechanism allowing plates to be loaded and unloaded. Trays of different heights (22, 47 and 77mm) allow the
stacking of up to four microtiter plates. The tray is suitable for all types of plates (deep well, low well, 24, 48 and 96 well).

 

 

Order Number Description Number of Microtiter Plates
SM3503.22 F-MT.22 24 - 48
SM3503.47 F-MT.47 24 - 96
SM3503.77 F-MT.77 24 - 144

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F-tray with rubber mat

Order number: SM3603

The whole tray is covered with a rubber mat and is particularly suitable for shaking containers at low speed (e.g. blood bags).

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F-tray with sticky mats

Order number: SMX350001

A tray fitted with sticky mats is especially useful for flasks shaken at low speeds (up to <200 rpm). The flask is placed directly on the sticky mat without the need for any other support. Flasks of all sizes can be used. Sticky mats are available individually or in sets and can also be supplied already fitted to a tray. The F-size tray accepts 7 mats. More information on the sticky mat options may be found here.

 

 

 

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F-tray with support bars

Order number: SM4130.6

This tray offers an alternative to both clamps and sticky mats and is ideal for tall containers. The tray is covered with a rubber mat and has an upright at each corner. The F-tray is fitted with six cross supports. These support bars securely clamp containers.

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F-tray for separating vessels

Kuhner has also designed a special tray for shaking separating vessels. This very flexible tray can take vessels of various sizes. It is also possible to add a second layer and double the capacity. Movable retaining rods allow vessels to be installed easily.

Order Number Description Number of separating vessels
SM430250 FS-250 10
SM4302502 FS-250/2 20 (2 layers)
SM430500 FS-500 8
SM4305002 FS-500/2 16 (2 layers)
SM431000 FS-1000/2 6
SM4310002 FS-1000/2 12 (2 layers)

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F-tray for centrifuge tubes

Order number: SM3805

The use of centrifuge tubes (e.g. 50ml Falcon tubes and TPP 50ml) in cell culture screening is well established. This F-tray has a capacity of 120 x 50 ml centrifuge tubes (5 racks of 24).

Holders for 600ml TPP tubes are also available.

 TPP tubes and plasticware available from:Midsci Logo

 

 

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Shelf

Order number: SMX1672

The ISF4-X incubator shakers can be fitted with a shelf allowing cultivation in petri dishes. One or two shelves can be placed above the shaking table and fixed to the chamber walls with four screws.

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ISIS-software

Kuhner developed the user friendly ISIS-Software for supervision, monitoring, and recording of data. The software is easy to operate.

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Interfaces

Kuhner shakers are equipped with a CAN-bus. CAN-USB, CAN-Ethernet, EMI-60 and NET-60 are all well established interfaces.

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FAQ

FAQ

Large incubator shaker with a minimal footprint and up to five independent shaker units.

 

? Is linear shaking available?
!

Yes, in addition to flexible orbital shaking diameters, Kuhner machines are capable of several linear shaking throws.

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? Is it possible to alter the height of the shaking units in an ISF4-X?
!

Yes, there are 37 possible positions and these may be changed at any time after delivery by the end user. It is not uncommon that users change shelf height to accomodate specific campaigns - then return the shakers to original position when workflow shifts to a different operation.

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?

How many shaking units will fit in an ISF4-X?

!

It depends on the height of the vessels you are shaking, but up to five shaker may be installed if using well plates.  Please contact Kuhner  for further information.

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Can the shaking diameter and speed of each shaking unit be set individually?

!

Yes, as with all Kuhner Shakers, the orbit diamter may be changed at any time after purchase. The machines remain entirely flexible after delivery.

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Is IQ-OQ documentation available for the shakers?

!

Yes, a full IQ-OQ package is available.

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?

Can the machine be used in the GMP sector?

!

Yes, manufacturing applications is a very common use of our machines.

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