ISF1-X (Climo Shaker) 

product

 

isf1-x gross

Product

Stackable incubator shaker for microbial, mammalian and plant cell applications.

Description

The heart of every development program is the cultivation step - do this step correctly, without failure, and the rest will follow.

The ISF1-X incubator shaker is suitable for applications in modern laboratories, research centers and production facilities. It offers a large shaking capacity with a small footprint and also many features allowing straightforward, safe handling of product.

backtop Top

Performance Characteristics

  • Up to three machines can be stacked

  • Large shaking capacity with small footprint

  • User - adjustable shaking diameters (6mm, 12.5mm, 19mm, 25mm, 50mm and 70mm)

  • Heating and cooling

  • CO2 and dual-sided humidity control options available

  • Every machine rigorously calibratred prior to shipment

backtop Top

Reliability pays

Be first to market with your products using the ISF1-X. All ISF1-X components are designed for durability and reliability.  Save timeline and opportunity costs by investing in a Kuhner shaker - the cost-effective investment backed with a five year warranty.

 backtop Top

A system that grows with your needs

Up to three ISF1-X shaking incubators can be stacked together for greater shaking capacity. Stacking is a simple procedure without the need for special tools or stacking kits. Each machine operates independently and can be equipped with cooling if required. For cell cultivation the ISF1-X is available with CO2. If using microtiter plates or particularly long cultivation times in shake flasks, the controlled humidity option will help reduce evaporation.

isf1-x qualitaet

backtop Top

The Kuhner direct drive cannot be matched

The maintenance-free direct drive is both long lasting and economical. The drive is protected from spills and the all stainless steel interior has a drain at the back for quick removal of any unwanted liquids. The incubator chamber can be cleaned with chemicals or sterilized using optional UV lighting. Kuhner's direct drive system allows the shaking diameter to be changed at any time as well as guaranteeing low energy consumption.

backtop Top

A safe system

An upward opening door fitted with a safety switch, means the ISF1-X is easy to load and unload. Automatic supervision of the mains voltage protects the shaker and in the event of a voltage spike the unit disconnects from the mains. The machine resumes operation at the set values once power is restored.

backtop Top

Innovation Partners

The ISF1-X sets today's standard for incubator shakers thanks to its technology and functionality. To keep it that way, Kuhner maintains close contact with well-known universities and experts. We constantly investigate new developments looking for opportunities to further optimize the design and performance of our machines.

backtop Top

 

technical data

isf1-x gross

Technical data

Stackable incubator shaker for microbial, mammalian and plant cell applications.

Technical data

  SMX1500 SMX1501 SMX1503 
Refrigerator   no yes  yes
Humidity control   no no yes 
Temperature minimum  ambient +10oC ambient - 15oC ambient -15o
Temperature maximum 80oC 80oC 80oC
Humidity maximum - - 85% r.h.
Power consumption < 1300W < 1500W < 2000W

 

 

 

 

 

 

 

 

 

backtop Top

Machine

Volume (empty)  350 l
Weight (with cooling)  210 kg
Illumination  Halogen (3 x 10W)
Operation menu in German, French, English, Italian, Spanish
Interface, standard CAN-Bus
Interface, optional USB, Ethernet, digital, analogue
Ambient temperature 10oC up to 35oC

 Dimensions (in mm)

dimennsions isf1x

backtop Top

Temperature

Setting, digital  0.1oC
Accuracy, absolute  +/- 0.30oC (37oC)
Principle of sensor  Pt-100
Power of heating 1000W
Power of cooling 155...270W
Air circulation

300m3/h

backtop Top 

Humidity

 

SMX1503

Max at 25...55°C 85% r.h.
Setting, digital 1% r.h.
Accuracy, absolute +/- 2% r.h.
Principle of sensor capacitive
Water refill

automatic

Water heater

300W

Door heater 100W

backtop Top

CO2

 

SMX1034

Principle of sensor Infrared, NDIR
Measuring range 0...20% CO2
Setting, digital 0.1% 
Accuracy, absolute +/- 0.42% at 20% CO2
Non-linearity <0.1%
Temperature range 5...60oC
CO2 supply max. 1 bar overpressure

backtop Top

Mains Connection

SMX1021 220-240V / 50-60 Hz
SMX1022 190-210V / 50-60 Hz
SMX1023 110-120V / 50-60 Hz
SMX1024 95-105V / 50-60 Hz

backtop Top

Shaking Unit

Tray, size F (800x420 mm)
Loading, maximum 25 kg
Setting, digital 1 rpm
Accuracy, absolute +/- 1 rpm
Timer 1s...999h
Acceleration controlled
Active brake adjustable
Stop on position adjustable

backtop Top

Shaking Motion(s)

Standard machines have adjustable orbit diameters and 12.5mm, 25mm and 50mm are included with every instrument. Optional 19mm (3/4") and 70mm orbit diameters may be added at any time, to any machine.

 Shaking motion Speed
* orbital, Ø 12.5mm 20...500rpm
* orbital, Ø 19mm 20...450rpm
* orbital, Ø 25mm 20...400rpm
* orbital, Ø 50mm 20...300rpm
* orbital,Ø 70mm 20...200rpm
* linear, Ø 12.5mm 20...400rpm
* linear, Ø 25mm 20...300rpm
* linear, Ø 50mm 20...200rpm
* can be changed  

backtop Top

 

 

Options

isf1-x gross

Options

Stackable incubator shaker for microbial, mammalian and plant cell applications.

 

Cooling

All Kuhner incubator shakers can be fitted with cooling. The refrigeration system is regulated by a fully digital PID control ensuring minimal energy consumption. The cooling is switched on only when required as determined by the chamber temperature and set point.

backtop Top

 

Humidity control

Humidity control is an important factor in successful fermentation. Evaporation from microtiter plates, or when cultivating in flasks for long periods (e.g. cell cultures), can be significantly reduced with humidification.

backtop Top

 

CO2 control

CO2 control is essential when working with mammalian cell cultures. Kuhner was the first company to manufacture and supply incubator shakers with CO2 control more than ten years ago and continues to offer this option.

backtop Top

Pull-out table

The pull-out table is designed for the ISF1-X and ISF4-X incubator shakers as well as the Rack System. This remarkable device makes loading and unloading trays much easier.

Ausziehtisch2

backtop Top

Dual table

The dual table is an easy and economical way of doubling the shaking capacity. The dual shaking table consists of two levels. Each level will accept an E, EX or F size tray. However, with the dual table the shaking speed is limited to a maximum of 200 rpm.

IMG 1138

backtop Top

 

Illumination unit for photosynthesis (LED)

The ceiling of any Kühner incubator shaker can be fitted with LED modules for the cultivation of phototrophic organisms. The control module allows full programming of night/day cycles and variable light intensity.

DSC00105

backtop Top

 

UV lamp

As in a biological safety cabinet, the chamber of a Kuhner incubator shaker can also be sterilized with an integrated UV lamp. The lamp is situated to maximize irradiation of the chamber and shaking table. The UV lamp has a clearly marked external switch and when the door is opened, the UV-lighting is automatically switched off.

DSC00109

backtop Top

 

Black window

Available for light sensitive medium or organisms. Any Kuhner incubator shaker can be delivered with blackened windows to prevent unwanted daylight or UV radiation inside the incubator.

backtop Top

 

Perspex cover

To prevent accidental operation of the controllers, the panel can be protected by a perspex cover.

backtop Top

IQ-OQ documentation

IQ-OQ (Installation Qualification and Operation Qualification) is an equipment qualification according to GMP procedure. Kuhner offers this service which can also be carried out at the customer’s premises.

backtop Top

 

BPM-60

BPM-60 (Bioprocess Monitoring) is a non-invasive, online measurement of dissolved oxygen and pH in an Erlenmeyer flask.

Continuous, recording of dissolved oxygen and pH

Dissolved oxygen and pH can be easily measured online using the BPM-60. The system allows for one or both parameters to be recorded in the same flask. The values of dissolved oxygen and/or pH can be monitored simultaneously in eight different flasks.

The continuous data logging, compared to conventional sample taking, is automatic and consistent. The recorded data can be analyzed to identify oxygen limitation or inhibition and therefore optimize yields in shaken flasks.

Furthermore, the BPM-60 assists in the production of reproducible starter cultures for use in fermenters (see PAT-initiative of the FDA).

Not on battery mode

An integrated socket at the front of the shaking table makes simple data communication and power supply possible without the risk of wiring breaking. With this technology no battery is required.

ISIS software for data logging

Kuhner ISIS Software is capable of data recording. In addition to logging dissolved oxygen and pH it is also possible to record all other shaker parameters such as shaking speed and CO2 concentration. A full audit trail is also stored for each shaker connected.

bpm collage

backtop Top

 

 

Accessories

isf1-x gross

Accessories

Stackable incubator shaker for microbial, mammalian and plant cell applications.

 

Universal tray FU

Order number: SM3003

 The universal tray can be fitted with clamps for Erlenmeyer flasks, test tube
holders, microtiter plates, or custom made holders to suit your requirements.
Rearranging these clamps and holders is quick and simple.

  • Clamps for Erlenmeyer flasks
  • Test tube holders
  • High capacity tube holders
  • Holders for deep well microtiter plates (Duetz System)
  • Sticky mats

backtop Top

 

F-tray (800 x 420mm) with fixed clamps

The clamps are riveted onto this tray and cannot be removed. It offers maximum stability and will often hold a larger number of flasks than an universal tray.

Order Number Description Number of Clamps
SM330025 F-25 ml 153
SM330050 F-50 ml 100
SM330100 F-100 ml 74
SM330125 F-125 ml 60
SM330150 F-150 ml 60
SM330200 F-200 ml 40
SM330250 F-50 ml 40
SM330300 F-300 ml 30
SM330500 F-500 ml 26
SM331000 F-1000 ml 16
SM331500 F-1500 ml 12
SM332000 F-2000 ml 9
SM333000 F-3000 ml 8
SM334000 F-4000 ml 5
SM335000 F-5000 ml 4

backtop Top

 

F-tray (800 x 420mm) for microtiter plates

Kuhner has designed trays specifically for microtiter plates. Handles at the front of the tray open and close the special clamping mechanism allowing plates to be loaded and unloaded. Trays of different heights (22, 47 and 77mm) allow the
stacking of up to four microtiter plates. The tray is suitable for all types of plates (deep well, low well, 24, 48 and 96 well).

Order Number Description Number of Microtiter Plates
SM3503.22 F-MT.22 24 - 48
SM3503.47 F-MT.47 24 - 96
SM3503.77 F-MT.77 24 - 144

backtop Top

F-tray with rubber mat

 

Order number: SM3603

The whole tray is covered with a rubber mat and is particularly suitable for shaking containers at low speed (e.g. blood bags).

backtop Top

 

F-tray with sticky mats

 

Order number: SM350001

A tray fitted with sticky mats is especially useful for flasks shaken at low speeds (up to <200 rpm). The flask is placed directly on the sticky mat without the need for any other support. Flasks of all sizes can be used. Sticky mats are available individually or in sets and can also be supplied already fitted to a tray. The F-size tray accepts 7 mats. Additional information on our trays with sticky mats is found here.

backtop Top

 

F-tray with support bars

 

Order number: SM4130.6

This tray offers an alternative to both clamps and sticky mats and is ideal for tall containers. The tray is covered with a rubber mat and has an upright at each corner. The F-tray is fitted with six cross supports. These support bars securely clamp containers.

backtop Top

 

F-tray (800 x 420mm) for separating vessels

Kuhner has also designed a special tray for shaking separating vessels. This very flexible tray can take vessels of various sizes. It is also possible to add a second layer and double the capacity. Movable retaining rods allow vessels to be installed easily.

Order Number Description Number of separating vessels
SM430250 FS-250 10
SM4302502 FS-250/2 20 (2 layers)
SM430500 FS-500 8
SM4305002 FS-500/2 16 (2 layers)
SM431000 FS-1000/2 6
SM4310002 FS-1000/2 12 (2 layers)

backtop Top

F-tray for centrifuge tubes

 

Order number: SM3805

The use of centrifuge tubes (e.g. 50ml Falcon tubes and TPP 50ml) in cell culture screening is well established. This F-tray has a capacity of 120 x 50 ml centrifuge tubes (5 racks of 24).

Holders for 600ml TPP tubes are also available.

   TPP tubes and Plasticware available from:Midsci Logo

 

backtop Top

 

 

Floor stand

 

Order numbers: SM1560 (2 x ISF1-X), SM1561 (1 x ISF1-X)

For a comfortable working height Kuhner offers floor stands for both the ISF1-X and LT-X incubator shakers. These are available in a choice of 400mm or 765mm high. 

backtop Top

 

 

Shelf

 

Order number: SMX1572

The ISF1-X incubator shakers can be fitted with a shelf allowing cultivation in petri dishes. One or two shelves can be placed above the shaking table and fixed to the chamber walls with four screws.

backtop Top

 

 

Water bath

 

Order numbers: SMX1532 (ISF1-W), SMX1533 (ISF1-X)

To reduce evaporation from shake flasks or microtiter plates a stainless steel water bath can be placed inside the incubator. This water bath is not fitted with an automatic water supply and must be topped up manually.

backtop Top

 

 

ISIS-software

Kuhner developed the user friendly ISIS-Software for supervision, monitoring, and recording of data. The software is easy to operate.

backtop Top

 

 

Interfaces

Kuhner shakers are equipped with a CAN-bus. CAN-USB, CAN-Ethernet, EMI-60 and NET-60 are all well established interfaces.

backtop Top

 

 

FAQ

isf1-x gross

FAQ

Stackable incubator shaker for microbial, mammalian and plant cell applications.

 

? Do you need a special kit for stacking incubator shakers?
!

No, Kuhner machines do not need kits or special tools for stacking. Full instructions are provided in the User Manual.

backtop Top

 

? Is linear shaking available?
!

Yes

backtop Top

 

?

Is it possible to stack ISF1-X and ISF-1-W incubator shakers together?

!

Yes, but please make sure you stack the W-series shakers on top of the X-series and not vice versa.

backtop Top

 

?

Is there a BPM-60 connection for the ISF1-X?

!

Yes, this is integrated into the shaking table and enables power supply and data transfer.

backtop Top

 

?

Is IQ-OQ documentation available for the shakers?

!

Yes

backtop Top

 

?

Can the machine be used in the GMP sector?

!

Yes

backtop Top