Duetz-System

Product

Product

Cultivation of bacterial strains, mutants and clone libraries.

Description

The Duetz-System is an integrated, easy-to-use, and cost-effective technology platform for the rapid handling and growth of large numbers of microbial strains, clonal libraries, and mutant banks in 24 or 96-well microtiter plates.

The system enables the growth in microplates of thousands of strains simultaneously without excessive repetitive handling: all handling is performed in parallel for sets of 24 or 96 strains. No robotic equipment is required. The system was originally developed and validated at the Institute of Biotechnology of the ETH Zurich.

Most often it is found that, for cultivations of 7-14 days, users employ the 'low evaporation' versions of the plate covers below. The most common cover uses 'spongey silicone' compression layers - but specialty layers featuring solid silicone and steel covers featuring retentition pins for robotics handling are also available. Contact us if you have questions regarding which cover is best suited for your well plates and application.

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Performance characteristics

An integrated, easy-to-use and cost-effective technology platform for the rapid handling and growth of large numbers of microbial strains, clonal libraries, mutant banks and cell lines

  • 4 main components:  cryo-replicator, cryo-replicator press, sandwich cover, cover clamps

  • Cost effective alternative to robot systems

  • Cultivation of thousands of strains

  • Same quality of growth as in Erlenmeyer flasks with respect to oxygen transfer rates and reproducibility

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Replicator and press

 
SMCR1000     Cryo-replicator
SMCR1100 Cryo-replicator press
   

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Sandwich covers

For 24 well MTPs:
SMCR1224

Universal Cover: 24 deepwell MTPs, (spongy silicone, 2.5mm holes)

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SMCR1224a

Universal Cover: 24 deepwell MTPs low evaporation, (spongy silicone, 1.2mm holes)

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SMCR1224b

Cover: 24 deepwell MTPs SMCR1424a, b, c, and d (2.5mm hole with retention pins)

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SMCR1224c

Cover: 24 deepwell MTPs 24 deepwell MTPs SMCR1424a, b, c, and d (1.2mm hole low evap. with retention pins)

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SMCR1224d

Cover for all types of 24 deepwell MTPs(solid silicone, 2.5 mm holes)


SMCR1224e

Cover for all types of 24 deepwell MTPs (solid silicone, 1.2mm holes)


SMCR1224f

Cover for 24 deepwell MTPs SMCR1424a, b, c, and d (solid silicone, pins, 2.5mm holes)


SMCR1224g

Cover for 24 deepwell MTPs SMCR1424a, b, c, and d (solid silicone, pins, 1.2 mm holes, low evaporation)


For high 24 well plates:

SMCR1224h

Cover for high 24 well plates (wells of 25 ml each), spongy silicone, 2.5 mm holes

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SMCR1224i

Cover for high 24 well plates (wells of 25 ml each), spongy silicone, 1.2 mm holes, low evaporation



For regular 24 well plates:

SMCR1324

Universal sandwich cover for 24-well MTPs

(with solid silicone, 19.5 mm hole distance, 2.5 mm holes)

 

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SMCR1324a

Universal sandwich cover for 24-well MTPs, low evaporation

(with solid silicone, 19.5 mm hole distance, 1.2 mm holes)

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SMCR1324b

Universal sandwich cover for 24-well MTPs

(with solid silicone, 18 mm hole distance, 2.5 mm holes)

SMCR1324c

Universal sandwich cover for 24-well MTPs, low evaporation

(with solid silicone, 18 mm hole distance, 1.2 mm holes)

SMCR1324d

Universal sandwich cover for 24-well MTPs, low evaporation

(with spongy silicone, 18 mm hole distance, 1.2 mm holes)

SMCR1324e

Universal sandwich cover for 24-well MTPs, low evaporation

(with spongy silicone, 19.5 mm hole distance, 1.2 mm holes)

For 24 roundwell MTP plates:

SMCR1524

Sandwich cover for polystyrene 24 low roundwell MTPs from Corning

 

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SMCR1524a

Sandwich cover for polystyrene 24 low roundwell MTPs from Corning (low-evaporation version of SMCR1524)

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For 48 deepwell MTPs:

SMCR1248

Sandwich cover for 48- deepwell MTPs (from DOT Scientific)

(with spongy silicone, 1.2 mm holes)

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For 96 deepwell MTPs:

SMCR1296

Sandwich cover for all types of 96 deepwell MTPs

(with spongy silicone, 1.5 mm holes)

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SMCR1296a

Sandwich cover for all types of 96 deepwell MTPs

(with solid silicone, 0.8 mm holes, low evaporation)

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SMCR1296b

Sandwich cover for Greiner 96 deepwell MTPs and SMCR1496a

(with spongy silicone, fixed with pins, 1.5 mm holes)

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SMCR1296c

Sandwich cover for Greiner 96 deepwell MTPs and SMCR1496a

(with solid silicone, fixed with pins, 0.8 mm holes, low evap.)

SMCR1306

Sandwich cover for polystyrene 6 lowwell MTPs

(with spongy silicone, 1.2 mm holes)

SMCR1396

Universal sandwich cover for 96-well MTPs

(with solid silicone, 0.8 mm holes)

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SMCR1396a

Universal sandwich cover for 96-well MTPs

(with spongy silicone, 1.5 mm holes)

SMCR1396b

Sandwich cover for SMCR1496a, SMCR1496c, and Greiner MTPs

(with solid silicone, fixed with pins, 0.8 mm holes, low evap.)

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SMCR1396c

Sandwich cover for SMCR1496a, SMCR1496c, and Greiner MTPs

(with spongy silicone, fixed with pins, 1.5 mm holes)

SMCR1596

Sandwich cover for polystyrene 96 low roundwell MTPs

(only suitable for MTPs with empty spaces between wells)

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SMCR1306

Sandwich cover for polystyrene 6 lowwell MTPs

(with spongy silicone, 1.2 mm holes)

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Replacement parts

SMCR0001

20x blue microfiber filter inlay for all types of

deepwell covers and universal covers

SMCR0002

20x green ePTFE filter inlay for all types of

deepwell covers and universal covers

SMCR0003

20x blue microfiber filter inlay for SMCR1524 and SMCR1524a covers

for Corning MTPs (SMCR1524)

SMCR0004

20x green ePTFE filter inlay for SMCR1524 and SMCR1524a covers

for Corning MTPs (SMCR1524)

SMCR0005

20x blue microfiber filter inlay for SMCR1596

SMCR0006

20x green ePTFE filter inlay for SMCR1596

SMCR0007

5x Replacement spongy silicone seal, 2.5 mm holes for sandwich cover

for 24 deepwell MTP (SMCR1224, SMCR1224b)

SMCR0007a

5x Replacement spongy silicone seal, 1.2 mm holes for sandwich

cover 24 well MTP (SMCR1224a, SMCR1224c, SMCR1324d)

SMCR0008

5x Replacement solid silicone seal, 2.5 mm holes, for

universal cover 24-well MTPs, 19.5 mm hole distance (SMCR1324)

SMCR0008a

5x Replacement solid silicone seal, 1.2 mm holes, for

universal cover 24-well MTPs, 19.5 mm hole distance (SMCR1324a)

SMCR0008b

5x Replacement solid silicone seal, 2.5 mm holes, for

universal cover 24-well MTPs, 18 mm hole distance (SMCR1324b)

SMCR0008c

5x Replacement solid silicone seal, 1.2 mm holes, for

universal cover 24-well MTPs, 18 mm hole distance (SMCR1324c)

SMCR0008d

5x Replacement spongy silicone seal, 1.2 mm holes, for

universal cover 24-well MTPs, 19.5 mm hole distance (SMCR1324e)

SMCR0009

5x Replacement spongy silicone seal for sandwich cover for

24 lowwell MTP (SMCR1524)

SMCR0009a

5x Replacement solid silicone seal for sandwich cover for

24 lowwell MTP, low evap. (SMCR1524a)

SMCR0010

Stainless steel foil with pinholes for sandwich cover for

24 lowwell MTP (SMCR1524a)

SMCR0011

5x Replacement spongy silicone seal for sandwich cover for

96 well MTP (SMCR1296, SMCR1296b, SMCR1396a, 1.5 mm holes)

SMCR0012

5x Replacement solid silicone seal for sandwich cover for

96 well MTP (SMCR1296a, SMCR1296c, SMCR1396, 0.8 mm holes)

SMCR0013

5x Replacement silicone seal for sandwich cover for

96 low roundwell MTP (SMCR1596)

SMCR0014

Stainless steel foil with pinholes for sandwich cover for

96 low roundwell MTP (SMCR1596)

SMCR0015

5x Replacement spongy silicone seal for universal sandwich cover for

6 lowwell MTP (SMCR1506)

SMCR0016

5x Replacement spongy silicone seal, 1.2 mm holes for

sandwich cover 48 well MTP (SMCR1248)

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Microplates

 

SMCR1424

Polypropylene square deepwell MTP

(re-usable, 24 wells of 11 ml volume, flattened, detoxified)

 

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SMCR1424a

Polypropylene square deepwell MTP (re-usable, 24 wells 11 ml, flattened, detoxified, with 4 cavities to fit covers with pins)

SMCR1424b

8 x white polystyrene square deepwell MTP

(disposable, 24 wells of 11 ml volume, gamma-irradiated)

 

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SMCR1424c

8 x transparent polystyrene square deepwell MTP

(disposable, 24 wells of 11 ml volume, gamma-irradiated)

 

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SMCR1424d

8 x grey polystyrene square deepwell MTP

(disposable, 24 wells of 11 ml volume, transparant bottom, gamma-irradiated)

 

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SMCR1424e

8 x white polystyrene square deepwell MTP

(disposable, 24 wells of 11 ml volume, transparant bottom, gamma-irradiated)

 

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SMCR1424f

14 x  Roundwell MTP Porvair, white walls, transparent bottom

(24 wells of 3 ml volume, with lid, gamma irradiated)

SMCR1496

Polypropylene square deepwell MTP

(re-usable, 96 wells of 2.4 ml volume, flattened, detoxified)

 

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SMCR1496a

Polypropylene square deepwell MTP (re-usable, 96 wells of 2.4 ml, flattened, detoxified, with 4 cavities to fit covers with pins)

 

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SMCR1496b

Polypropylene square deepwell MTP

(re-usable, Greiner, 96 wells of 2.4 ml volume, flattened)

 

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SMCR1496c

20x transparent polystyrene square half deepwell MTP

(96 wells of 1.1 ml volume, gamma-irradiated)

 

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SMCR1496d

16x white polystyrene square half deepwell MTP (transparent polystyrene bottom, 96 wells of 1.1 ml volume, gamma-irradiated)

SMCR1496e

16x white polystyrene square half deepwell MTP

(transparent glass bottom, 96 wells of 1.1 ml volume, gamma-irradiated)

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Clamp systems

SMCR1600a

Clamp for 6-8 polystyrene lowwell MTPs

(fits Kuhner shaker platforms only)

SMCR1601

Clamp for 6-8 lowwell microtiter plates in gastight box

(fits Kuhner shaker platform)

SMCR1602a

Clamp for 2 PreSens SDR units + MTPs

(fits Kuhner shaker platforms only)

SMCR1700a

Cover clamp for 4 deepwell MTPs

(fits Kuhner shaker platforms only)

 

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SMCR1702a

Cover clamp for 2 deepwell MTPs

(fits Kuhner shaker platforms only)

SMCR1800a

Universal Clamp for 12-16 lowwell MTPs or 4 deepwell MTPs

(fits Kuhner shaker platforms only)

 

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SMCR1801a

Extra high universal clamp with 4 positions, also suitable for MTPs with 24 wells of 25 ml (fits Kuhner shaker platforms only)

 

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SMCR1802a

Clamp for 4 PreSens SDR units + MTPs

(fits Kuhner shaker platforms only)

SMCR1803a

Universal Clamp with zero-evaporation box on top

(fits Kuhner shaker platforms only)

SMCR0017

EPDM rectangular sealing for gastight box

(Replacement part for SMCR1803a)

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Sets of products

SMCR7024

4x sandwich cover for 24 deepwell MTPs (SMCR1224)

4x square deepwell microtiter plate (SMCR1424)

1x cover clamp for 4 deepwell MTPs (SMCR1700a)

SMCR7024b

4x sandwich cover for polystyrene 24 deepwell MTPs

(with pins at the inside, fixing the silicone, SMCR1224b)

1x cover clamp for 4 deepwell MTPs (SMCR1700a)

SMCR7096

4x sandwich cover for 96 deepwell plate (SMCR1296)

4x square deepwell microtiter plate (SMCR1496)

1x cover clamp for 4 deepwell MTPs (SMCR1700a)

SMCR7096b

4x sandwich cover for 96 deepwell MTP (SMCR1296b)

4x square deepwell MTPs Greiner (SMCR1496b)

1x cover clamp for 4 deepwell MTPs (SMCR1700a)

SMCR8024

12x sandwich covers 24 lowwell MTPs (SMCR1524)

1x clamp for 12-16 lowwell MTPs (SMCR1800a)

SMCR8096

16x sandwich covers 96 lowwell MTPs (SMCR1596)

1x clamp for 12-16 lowwell MTPs (SMCR1800a)

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